Bonita G. Taffe

Professional Experience:

2002      Diplomate of the American Board of Toxicology
1999-      Assistant Professor, Department of Occupational and Environmental Health Sciences,       Wayne State University, Detroit, MI.
1997 -        Member, EHS Center for Molecular and Cellular Toxicology
1995 -         Member, Michigan Cancer Foundation/Currently Karmanos Cancer Institute
1994-          Adjunct Assistant Professor, Department of Pharmaceutical Sciences, Wayne State University,         Detroit, MI.
1993- 1999 Assistant Professor, Research, Department of Occupational and Environmental Health Sciences,        Wayne State University, Detroit, MI.
1992-1993  Senior Staff Fellow, Laboratory of Molecular Genetics, National Institute on Aging, Baltimore, MD
1991-1992  Senior Staff Fellow, Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, MD..
1990-1991  Visiting Scientist, Foundation for the Promotion of Cancer Res., National Cancer Center Research     Institute Tokyo, Japan.
1988-1990  IRTA Fellow, Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, MD.
1980-1982  Research Assistant, Department of Family Medicine, Division of Occupational and Environmental     Health Sciences, Mount Sinai School of Medicine, New York, NY. 
1977-1979  Research Assistant, Department of Pulmonary Medicine, Yale University, New Haven, CT.
1976-1977  Assistant in Research, University of Connecticut, Farmington, CT.

University of Connecticut, Storrs, CT
Columbia U. School of Public Health, New York,  NY      
Johns Hopkins School of Hygiene and Public Health,
             Baltimore, MD  B.S.                 
M.P.H.             
Ph.D. 1976
1981
1988 Biology
Env. Health Sciences
Toxicology

Wolff, M. S., Taffe, B., Boesch, R. R. and Selikoff, l. J.: Detection of polycyclic aromatic hydrocarbons in skin oil obtained from roofing workers. Chemosphere 11: 595 599,1982.
Kensler, T. W., Egner, P. A., Moore, K. G., Taffe, B. G., Twerdok, L. E. and Trush, M. A.: Role of inflammatory cells in the metabolic activation of polycyclic aromatic hydrocarbons in mouse skin. Toxicol. Appl. Pharmacol. 90: 337 346,1987.
Taffe, B. G., Takahashi, N., Kensler, T. W. and Mason, R. P.: Generation of free radicals from organic hydroperoxide tumor promoters in isolated mouse keratinocytes. J. Biol. Chem.262: 12143 12149,1987.
Taffe, B. G. and Kensler, T. W.: Tumor promotion by a hydroperoxide metabolite of butylated
hydroxytoluene,2,6 di tert butyl 4 hydroperoxy4 methyl 2,5 cyclohexadienone in mouse skin.  Res. Commun. Chem. Pathol. Pharmacol. 61: 291 303,1988.
Taffe, B. G., Zweier, J. L., Pannell, L. R. and Kensler, T. W.: Generation of reactive intermediates from the tumor promoter butylated hydroxytoluene hydroperoxide in isolated murine keratinocytes or by hematin. Carcinogenesis 10: 1261 1268,1989.
Matsukura, N., Willey, J., Miyashita, M., Taffe, B., Waldren, C., Puck, T. T. and Harris, C. C.: Detection of direct mutagenicity of cigarette smoke condensate in mammalian cells. Carcinogenesis 12: 685 689, 1991 .
Evans, M.K., Taffe, B.G., Harris, C.C. and Bohr, V.A.: DNA strand bias in the repair of the p53 gene in normal human and Xeroderma pigmentosum group C fibroblasts.  Cancer Res. 53: 5377-5381, 1993.
Wang, X.W., Yeh, H., Scheffer, L., Roy, R., Moncollin, V., Egly, J.M., Wang, Z., Freidberg, E.C., Evans, M.K., Taffe, B.G.: p53 modulation of TFII H-associated nucleotide excision repair activity. Nature Genetics, 10:188-195, 1995.
Taffe, B.G., Larminat, F.L.,Laval, J., Croteau, D., Anson, M., Bohr, V.A.: Gene specific nuclear and mitochondrial  repair of formamidopyrimidine DNA Glycosylase-sensitive sites in Chinese hamster ovary cells, Mut. Research,  364, 183-92, 1996.
Joshi, B., Li, L., Taffe, B.G., Zhu, Z., Wahl, S., Tian, H., Ben-Josef, E., Taylor, J.D., Porter, A.T., Tang, D.G.: Apoptosis induction by a novel anti-prostate cancer compound, BMD188 (a fatty acid-containing hydroxamic acid), requires the mitochondrial respiratory chain\", Cancer Res. 59:4343-4355, 1999.
Djuric, Z., Potter, D.W., Taffe, B.G., Strasburg, G..M., \"Effect of iron and hydrogen peroxide on DNA and lipid oxidation\", J. Biochem. Molec. Tox. 15(2):114-119, 2001.
Dolgachev, V, Nagy, B, Taffe, B, Hanada, K, Separovic, D, Reactive oxygen species generation is independent of the de novo sphingolipids in apoptotic photosensitized cells, Journal of Experimental Cell Research, Aug 15; 288(2):425-36.

Papers Published in Conference Proceedings:

Taffe, B. G., Kensler, T. W., Takahashi, N. and Mason, R. P.: Activation of organic hydroperoxide tumor promoters to free radicals in target cells. In Cerutti, P. A., Nygaard, O. F. and Simic, M. G. (Eds.): Anticarcinogenesis and Radiation Protection. New York, Plenum Press, 1987, pp. 191 197.
Taffe, B. G., Zweier, J. L. and Kensler, T. W.: Tumor promotion by butylated hydroxytoluene hydroperoxide: Role for free radicals? In Kabara, J. J. (Ed.): The Pharmacological Effects of Lipids lll: Role of Lipids in Carcinogenesis and Therapv. Illinois, American Oil Chemists Society, 1989, pp. 293 302.
Wilson, VL, Taffe, BG, Shields, PG, Povey, AC, Harris, CC. Detection and quantification of 8-hydroxydeoxyguanosine adducts in peripheral-blood of people exposed to ionizing radiation. Environ. Health Perspect. 99;261-263, 1993.
Bohr, V.A., Taffe, B.G. and Larminat, F.: DNA repair, oxidative stress and aging. In Cutler, R., Packer, L., Bertram, J and Mori, A. (Eds.): Oxidative Stress and Aging, Birkhauser Verlag, Basel, Switzerland, 1995.

Abstracts

Taffe, B.G., Evans, M.K., Harris, C.C, Bohr, V A.: Repair in specific genes mutated during tumorigenesis. Proc. Am. Assoc. Cancer Res. 33:180,1992.
Taffe, B.G., Larminat, F., Laval, J., Anson, M., Bohr, V.A.: Gene specific repair of 8-oxodeoxyguanosine DNA Adducts.  AACR Special Conference \"Risk Assessment in Environmental Carcinogenesis\",1994.
Evans, M.K., Taffe, B.G., Harris, C.C., Bohr, V.A.: DNA repair in the p53 tumor suppressor gene in Li-Fraumeni syndrome and other cancer prone disorders.   Proc. Am. Assoc. Cancer Res. 35:638, 1994.
Taffe, B.G., Larminat, F.L.,Laval, J., Anson, M., Bohr, V.A.: Lack of gene specific repair of 8-oxogeoxyguanosine DNA adducts. Proc. Am. Assoc. Cancer Res., 36;849, 1995.
Tobalina, G., Taffe, B.G., Effect of Chromium (III) Picolinate on Mitochondrial DNA, National Minority Research Symposium, New Orleans, LA, 1997.
Taffe, B.G., Karpinski, S.: Evaluation of methods for mitochondrial DNA isolation and analysis.  Proc. Am. Assoc. Cancer Res., 39:1639, 1998.
Tobalina, G., Campbell, C., Taffe, B.G., Effects of Chromium (III) Picolinate on intact mitochondria, National Minority Research Symposium, New York, NY, 1998.
Peng, H., Land, S., Taffe, B.G., Development of a fluorescent Real Time polymerase chain reaction (PCR) assay to quantitate mouse mitochondrial deletions, Proc. Am. Assoc. Cancer Res., 42;645, 2001.

Wolff, M. S., Taffe, B., Boesch, R. R. and Selikoff, l. J.: Detection of polycyclic aromatic hydrocarbons in skin oil obtained from roofing workers. Chemosphere 11: 595 599,1982.
Kensler, T. W., Egner, P. A., Moore, K. G., Taffe, B. G., Twerdok, L. E. and Trush, M. A.: Role of inflammatory cells in the metabolic activation of polycyclic aromatic hydrocarbons in mouse skin. Toxicol. Appl. Pharmacol. 90: 337 346,1987.
Taffe, B. G., Takahashi, N., Kensler, T. W. and Mason, R. P.: Generation of free radicals from organic hydroperoxide tumor promoters in isolated mouse keratinocytes. J. Biol. Chem.262: 12143 12149,1987.
Taffe, B. G. and Kensler, T. W.: Tumor promotion by a hydroperoxide metabolite of butylated
hydroxytoluene,2,6 di tert butyl 4 hydroperoxy4 methyl 2,5 cyclohexadienone in mouse skin.  Res. Commun. Chem. Pathol. Pharmacol. 61: 291 303,1988.
Taffe, B. G., Zweier, J. L., Pannell, L. R. and Kensler, T. W.: Generation of reactive intermediates from the tumor promoter butylated hydroxytoluene hydroperoxide in isolated murine keratinocytes or by hematin. Carcinogenesis 10: 1261 1268,1989.
Matsukura, N., Willey, J., Miyashita, M., Taffe, B., Waldren, C., Puck, T. T. and Harris, C. C.: Detection of direct mutagenicity of cigarette smoke condensate in mammalian cells. Carcinogenesis 12: 685 689, 1991 .
Evans, M.K., Taffe, B.G., Harris, C.C. and Bohr, V.A.: DNA strand bias in the repair of the p53 gene in normal human and Xeroderma pigmentosum group C fibroblasts.  Cancer Res. 53: 5377-5381, 1993.
Wang, X.W., Yeh, H., Scheffer, L., Roy, R., Moncollin, V., Egly, J.M., Wang, Z., Freidberg, E.C., Evans, M.K., Taffe, B.G.: p53 modulation of TFII H-associated nucleotide excision repair activity. Nature Genetics, 10:188-195, 1995.
Taffe, B.G., Larminat, F.L.,Laval, J., Croteau, D., Anson, M., Bohr, V.A.: Gene specific nuclear and mitochondrial  repair of formamidopyrimidine DNA Glycosylase-sensitive sites in Chinese hamster ovary cells, Mut. Research,  364, 183-92, 1996.
Joshi, B., Li, L., Taffe, B.G., Zhu, Z., Wahl, S., Tian, H., Ben-Josef, E., Taylor, J.D., Porter, A.T., Tang, D.G.: Apoptosis induction by a novel anti-prostate cancer compound, BMD188 (a fatty acid-containing hydroxamic acid), requires the mitochondrial respiratory chain\", Cancer Res. 59:4343-4355, 1999.
Djuric, Z., Potter, D.W., Taffe, B.G., Strasburg, G..M., \"Effect of iron and hydrogen peroxide on DNA and lipid oxidation\", J. Biochem. Molec. Tox. 15(2):114-119, 2001.
Dolgachev, V, Nagy, B, Taffe, B, Hanada, K, Separovic, D, Reactive oxygen species generation is independent of the de novo sphingolipids in apoptotic photosensitized cells, Journal of Experimental Cell Research, Aug 15; 288(2):425-36.

Papers Published in Conference Proceedings:

Taffe, B. G., Kensler, T. W., Takahashi, N. and Mason, R. P.: Activation of organic hydroperoxide tumor promoters to free radicals in target cells. In Cerutti, P. A., Nygaard, O. F. and Simic, M. G. (Eds.): Anticarcinogenesis and Radiation Protection. New York, Plenum Press, 1987, pp. 191 197.
Taffe, B. G., Zweier, J. L. and Kensler, T. W.: Tumor promotion by butylated hydroxytoluene hydroperoxide: Role for free radicals? In Kabara, J. J. (Ed.): The Pharmacological Effects of Lipids lll: Role of Lipids in Carcinogenesis and Therapv. Illinois, American Oil Chemists Society, 1989, pp. 293 302.
Wilson, VL, Taffe, BG, Shields, PG, Povey, AC, Harris, CC. Detection and quantification of 8-hydroxydeoxyguanosine adducts in peripheral-blood of people exposed to ionizing radiation. Environ. Health Perspect. 99;261-263, 1993.
Bohr, V.A., Taffe, B.G. and Larminat, F.: DNA repair, oxidative stress and aging. In Cutler, R., Packer, L., Bertram, J and Mori, A. (Eds.): Oxidative Stress and Aging, Birkhauser Verlag, Basel, Switzerland, 1995.

Abstracts

Taffe, B.G., Evans, M.K., Harris, C.C, Bohr, V A.: Repair in specific genes mutated during tumorigenesis. Proc. Am. Assoc. Cancer Res. 33:180,1992.
Taffe, B.G., Larminat, F., Laval, J., Anson, M., Bohr, V.A.: Gene specific repair of 8-oxodeoxyguanosine DNA Adducts.  AACR Special Conference \"Risk Assessment in Environmental Carcinogenesis\",1994.
Evans, M.K., Taffe, B.G., Harris, C.C., Bohr, V.A.: DNA repair in the p53 tumor suppressor gene in Li-Fraumeni syndrome and other cancer prone disorders.   Proc. Am. Assoc. Cancer Res. 35:638, 1994.
Taffe, B.G., Larminat, F.L.,Laval, J., Anson, M., Bohr, V.A.: Lack of gene specific repair of 8-oxogeoxyguanosine DNA adducts. Proc. Am. Assoc. Cancer Res., 36;849, 1995.
Principal Investigator: B. G. Taffe
Agency: NIH/NCI
Type: R29  Period: 5/1/97-4/31/02

The major objective of this project is to characterize, quantitate and compare nitric oxide-mediated DNA damage in isolated DNA, isolated mitochondria, and intact cells and to measure DNA repair in mitochondrial and nuclear DNA of cultured cells.

“Bleomycin Damage to Mitochondria in proliferating lung cells

Principal Investigator: B. G. Taffe
Agency: Michigan Chapter American Lung Association
Type: Research grant  Period: 10/97-9/99

The major goal of this project was to determine whether differential responses to oxidant stress occurred between human fibroblasts and endothelial cells, and whether differences in cytotoxicity related to mitochondrial damage and/or the cellular capacity for glycolytic metabolism.

Principal Investigator: B. G. Taffe
Agency: Pharmaceutical Research Manufacturers of America
Type: Starter grant  Period: 1/95-1/97
The major goal of this project was to develop models and methodology to study DNA damage mediated by NO in cells and to characterize repair of this damage.